Gel Permeation Chromatography (GPC)
Size Exclusion Chromatography (SEC)

GPC/SEC separation mechanism

SEC/GPC is a chromatography based separation technique where no interaction occurs between the stationary phase and the sample. Due to the porous column material molecules with a smaller hydrodynamic volume exhibit a longer retention time. Thus, the larger the molecule the faster the elution. SEC/GPC is a relative method since each sample needs to be compared to standards of known molecular weight and elution time.


Determination of:

- Number average molecular weight Mn
- Weight average molecular weight Mw
- Molecular weight distribution/dispersity Ð = Mw / Mn


Equipment:

- several systems working on different solvents (aqueous/organic) with UV/RI detection
- system equipped with molar mass sensitive detectors (MALLS, viscosity)


Literature:

1.
D. Berek
Size exclusion chromatography - A blessing and a curse of science and technology of synthetic polymers
Journal of Separation Science, 2010, 33, 315
2.
M. Gaborieau, P. Castignolles
Size exclusion chromatography (SEC) of branched polymers and polysaccharides
Analytical and Bioanalytical Chemistry, 2011, 399, 1413
3.
L. K. Konstanski, D. M. Keller, A. E. Hamielec
Size exclusion chromatography - a review of calibration methodologies
Journal of Biochemical and Biophysical Methods, 2004, 58, 159-186
4.
A. M. Striegel, W. W. Yau, J. J. Kirkland, D. D. Bly
Modern Size-Exclusion Liquid Chromatography
J. Wiley & Sons, Hoboken, 2009

Contact:   Sandra Seywald Ute Heinz Christine Rosenauer
Phone: 06131-379 - 227       06131-379 - 227       06131-379 - 225
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