Biomacromolecules such as peptides, proteins and DNA have emerged as an important class of therapeutics. In 2017, biologics comprise about 20% of total global pharmaceutical spending. The syntheses and optimization of such molecules by the cellular machinery are limited. Hence, we seek to develop new synthesis pathways to modify biomolecules in a site-directed fashion to prepare a new generation of structurally precise protein constructs which could not be achieved by molecular biology methods alone. The multicomponent protein complexes with improved features will be employed to address current challenges for biomedical applications such as new administration routes, formulations with higher efficacy or programming stimuli responsiveness.
Protein-Drug Conjugates, Bioactive Peptides and Proteins
Lujuan Xu, Marco Raabe, Maksymilian Marek Zegota, João C. F. Nogueira, Vijay Chudasama, Seah Ling Kuan, and Tanja Weil, "Site-selective protein modification via disulfide rebridging for fast tetrazine/trans-cyclooctene bioconjugation," Organic & Biomolecular Chemistry 18 (6), 1140-1147 (2020).
Maksymilian Marek Zegota, T. Wang, Christiane Seidler, David Yuen Wah Ng, Seah Ling Kuan, and Tanja Weil, ""Tag and Modify" Protein Conjugation with Dynamic Covalent Chemistry," Bioconjugate Chemistry 29 (8), 2665-2670 (2018).
Supramolecular Fusion Proteins, Multimodal Therapy, Chemiluminescent Hybrids
Image adapted from Chem. Eur. J. 2016, 22, 17112; © 2016 Wiley-VCH Verlag
Astrid Heck, Theresa Ostertag, Leonie Schnell, Stephan Fischer, Bikram Keshari Agrawalla, Pia Winterwerber, Eva Wirsching, Michael Fauler, Manfred Frick, Seah Ling Kuan, Tanja Weil, and Holger Barth, "Supramolecular Toxin Complexes for Targeted Pharmacological Modulation of Polymorphonuclear Leukocyte Functions," Advanced Healthcare Materials 8 (17), 1900665 (2019).
Seah Ling Kuan, Fernando, R. G. Bergamini, and Tanja Weil, "Functional protein nanostructures: a chemical toolbox," Chemical Society Reviews 47 (24), 9069-9105 (2018).
Seah Ling Kuan, S. Fischer, S. Hafner, T. Wang, T. Syrovets, Weina Liu, Yu Tokura, David Yuen Wah Ng, A. Riegger, C. Förtsch, D. Jäger, T. F. E. Barth, T. Simmet, H. Barth, and Tanja Weil, "Boosting Antitumor Drug Efficacy with Chemically Engineered Multidomain Proteins," Advanced Science 5 (8), 1701036 (2018).
Biohybrids, Drug Delivery, Antimicrobial Therapy, Photodynamic Therapy
S. Obuobi, Y. Wang, J. S. Khara, Andreas Riegger, Seah Ling Kuan, and P. L. R. Ee, "Antimicrobial and Anti-Biofilm Activities of Surface Engineered Polycationic Albumin Nanoparticles with Reduced Hemolytic Activity," Macromolecular Bioscience 18 (10), 1800196 (2018).
Seah Ling Kuan, B. Stöckle, Jörg Reichenwallner, David Y. W. Ng, Y. Z. Wu, Mikheil Doroshenko, Kaloian Koynov, Dariush Hinderberger, Klaus Müllen, and Tanja Weil, "Dendronized Albumin Core-Shell Transporters with High Drug Loading Capacity," Biomacromolecules 14 (2), 367-376 (2013).
"If I have seen further it is by standing on the shoulders of Giants."
― Sir Isaac Newton